Data treatment/pre-processing¶
- Quality
- Filtering Reads
- Trimming Reads
- Contamination
- Barcodes / multiplexing
- Read Merging
Learning Objectives¶
- Assess the overall quality of NGS (FastQ format) sequence reads
- Visualise the quality, and other associated matrices, of reads to decide on filters and cutoffs for cleaning up data ready for downstream analysis
- Clean up adaptors and pre-process the sequence data for further analysis